Stable Isotope Probing and Related Strategies
by Murrell, J. Colin; Whiteley, Andrew S.Format: Hardcover
Pub. Date: 2010-12-31
Publisher(s): Amer Society for Microbiology
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Summary
Stable isotopes, isotopes that are non-reactive, are chemically very similar and thus their behavior is close to identical, but fractionation technologies can separate the isotopes because of their different mass. They have been used for a number of years in botanical studies, but more recently stable isotopes-chiefly oxygen, nitrogen and carbon- have been used in ecological and climate studies. This volume contains chapters by leading researchers in microbial ecology who have been using and developing stable isotope technology, often in combination with genomics. It offers a timely look at the use of stable isotopes in many microbial ecology applications, which has ballooned in the last few years.
Table of Contents
| Contributors | p. vii |
| Preface | p. xi |
| Methodology | |
| DNA Stable Isotope Probing | p. 3 |
| RNA Stable Isotope Probing | p. 25 |
| Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology | p. 37 |
| Protein Stable Isotope Probing | p. 73 |
| Stable Isotope Probing and Metagenomics | p. 97 |
| Stable Isotope Probing Techniques Using H218O | p. 115 |
| Stable Isotope Probing Techniques Using 15N | p. 129 |
| Applications of Stable Isotope Probing | |
| Stable Isotope Probing and Plants | p. 151 |
| Stable Isotope Probing Techniques and Bioremediation | p. 165 |
| Trophic Interactions in Microbial Communities and Food Webs Traced by Stable Isotope Probing of Nucleic Acids | p. 203 |
| Stable Isotope Probing and the Human Gut | p. 233 |
| DNA Stable Isotope Probing and Gene Mining | p. 259 |
| Related Stable Isotope Techniques | |
| Raman-Fluorescence In Situ Hybridization | p. 279 |
| Nano-Secondary Ions Mass Spectrometry (nanoSIMS) Coupled with In Situ Hybridization for Ecological Research | p. 295 |
| FISH-Microautoradiography and Isotope Arrays for Monitoring the Ecophysiology of Microbes Within Their Natural Environment | p. 305 |
| RNA-Radioisotope Probing for Studying Carbon Metabolism in Soils | p. 317 |
| A Glance Toward the Future: Where Do We Go from Here? | p. 333 |
| Index | p. 337 |
| Table of Contents provided by Ingram. All Rights Reserved. |
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